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National Agricultural Laboratory in São Paulo (LANAGRO-SP)


 Contact person in the laboratory

 Maria de Fátima Martins Pinhel

 Email of contact person

 This email address is being protected from spambots. You need JavaScript enabled to view it.

 Mandate of the Laboratory:

 LANAGRO-SP is an Official Laboratory of MAPA - Ministry of   Agriculture, Livestock and Food Supply. It is to support, through   laboratory analysis, the inspection actions and monitoring programs   of the SDA - Agriculture and Livestock Defense Secretariat.

 Role in the farm-to-fork chain:

 The main role is to support MAPA’s policies such as to coordinate   and monitor technical studies, science and technology, analytical   tests and actions by trace, control, monitoring and evaluation   processes relating to the safety and quality of animal and vegetal   origin products, and animal health.

 National Reference Laboratory:

   Yes. LANAGRO-SP is an OIE reference laboratory for avian     influenza  and Newcastle disease diagnosis. As a national l   laboratory,  LANAGRO-SP also acts according to the MAPA’s policies   in official inspection programs, analysing products of animal origin,   animal feed, beverages, fertilizers, avian vaccines, anti- rabies   vaccines, veterinary drugs, pesticides and samples for avian   diagnosis, in order to ensure the safety and quality of the product for   domestic market, exporting and importing operations. Besides that,   LANAGRO- SP is reference laboratory to the PNCRC – National   Program for Residues and Contaminants Control, processing   samples of meat (poultry, cattle, swine, equine, and fish) and milk,   for exportation, importation and national trade. Other activities   includes the participation on audits in private laboratories of the   MAPA’s Network laboratories and research, development and   validation of laboratory methods.

 Type of contaminants analysed:

 ANIMAL AREA
 Residues of Veterinary Drugs
 Residues of Inorganic Contaminants
 Residues of Pesticides: Organochlorines pesticides and PCBs
 Microbial contaminants
 PLANT AREA
 Microbial contaminants

 Matrices analysed:

 Matrices of animal origin: meat of bovine, equine, swine, fish and   shrimp, poultry, ostrich, sheep, goat, rabbit; honey and milk.
 Matrices of plant area: beverages

 Combination matrices and contaminants
 analysed:

See attached table – Contaminants and Matrices/Species -
LANAGRO-SP 

 Description of validated method used:

Inorganic Contaminants Residues
- Determination of cadmium and lead (Cd and Pb) in meat by graphite furnace atomic absorption spectrometry (GF AAS)
-Determination of the inorganic contaminant arsenic (As) in meat by atomic absorption spectrometry with generation of hydrides (HG AAS)
- Determination of mercury in fish and meat by TDA AAS
- Determination of inorganic contaminants in fresh milk by ICP-MS

Organochlorine pesticide residues
- Determination of organochlorines pesticides and PCBs in animal fat by CG – ECD and CG-MS
- Determination of organochlorines pesticides and PCBs in fish muscle by CG – ECD and CG-MS

Veterinary drugs residues
- Determination of chloramphenicol in honey by LC-MS / MS
- Determination of thyrostatics in swine urine by LC-MS / MS
- Determination of ractopamine in swine urine by LC-MS / MS
- Determination of nitrofurans metabolites in poultry muscle by LC-MS / MS
- Determination of nitrofurans metabolites in equine muscle by LC-MS / MS
- Determination of nitrofurans metabolites in shrimp muscle by LC-MS / MS
- Determination of β-agonists in bovine urine by LC-MS / MS
- Determination of β-agonists in bovine and porcine muscle by LC-MS / MS

Microbial contaminants
-Presumptive detection of Salmonella spp. by polymerase chain reaction (AFNOR Validation nº BIO 18/03-11/02)
- Detection of Salmonella spp. (ISO 6579-1:2017)
- Coliform Count (ISO 4832:2006)
- Thermotolerant coliforms Count
- Most Likely Number of Coliforms (ISO 4831:2006)
- Detection, isolation and identification of Escherichia coli O157:H7 (MLG 5.09 e MLG 5A.04)
- Presumptive detection of Listeria monocytogenes by polymerase chain reaction (AFNOR Validation QUA 18/05-07/08)
- Presumptive count of Bacillus cereus (ISO 7932:2004)
- Count of Clostridium perfringens (ISO 7937:2004)
- Count of Staphylococcus aureus (BAM:2016)
- Detection and isolation of Escherichia coli producing Shiga toxin (STEC) non O157
(MLG 5B.05)
- Yeast and mold counts in products with water activity greater than 0,95 (ISO 21527-1:2008)
- Yeast and mold content in products with water activity less than or equal to 0,95 (ISO 21527-2:2008)

 Laboratory accredited according to
 ISO17025:

Yes 

 Provision of interpretation of residue
 data to customers:

 Yes

 Laboratory certified according to GLP:

 No

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